| System | NANOSPACE SI-2, OSAKA SODA, JAPAN |
| Column | CAPCELL PAK C18 SCX UG80 (4.6 ×150 mm, 5μm), OSAKA SODA, JAPAN |
| Mobile phase | 5mM KH PO (pH 2.5, adjusted with phosphoric acid) / MeOH (9 : 1) |
| Flow rate | 1.0 mL/min |
| Temperature | 40°C |
| Detection | PDA (280 nm) |
| Inj. vol. | 10 µL |
| Sample dissolved in | DW |